Association between IL1R1 rs2234650 Polymorphism in Patients with Acute Lymphoblastic Leukemia Infected with HHV-6A

Maryam Sabri Ibrahim; Halah Dawood Salman; Aya Raed Alheany; Shakir Hammad Al-Alwany

doi:10.54133/ajms.v8i2.1705

Authors

Maryam Sabri Ibrahim Department of Medical Microbiology, College of Medicine, Mustansiriyah, University, Baghdad, Iraq https://orcid.org/0000-0003-4044-512X
Halah Dawood Salman Department of Clinical Lab Sciences, College of Pharmacy, University of Babylon, Babylon, Iraq https://orcid.org/0000-0001-5917-7992
Aya Raed Alheany College of Medicine, Ibn Sina University of Medical and pharmaceutical Sciences, Baghdad, Iraq
Shakir Hammad Al-Alwany College of Science, University of Babylon, Babylon, Iraq

DOI:

https://doi.org/10.54133/ajms.v8i2.1705

Keywords:

Acute lymphoblastic leukemia, Human herpesvirus 6-A, Interleukin-1 receptor type 1, rs2234650, PCR, Sequencing

Abstract

Background: Everyone contracts the common infection known as human herpesvirus 6 (HHV-6). Particularly associated with encephalitis, human herpesvirus 6B (HHV-6B) poses a risk of morbidity and death to recipients of allogeneic hematopoietic stem cell transplants. IL-1β and interleukin-1α (IL-1α) are critical for enhancing infection resistance. Objective: To ascertain whether HHV-6A and the IL1R1 rs2234650 gene polymorphism are related and could be risk factors for Iraqi infants developing acute lymphoblastic leukemia (ALL). Methods: This case-control study included 150 blood samples from 100 patients diagnosed with ALL and 50 from healthy subjects. The IL1R1 rs2234650 gene polymorphism was identified using the Sanger sequencing method, and HHV-6A using PCR. Results: Age group and sex were found to differ significantly. Of 100 samples, 32 percent had HHV6A. The polymorphism of the IL-1R1 rs2234650 gene revealed that the distribution of DNA polymorphisms according to C\C, C\G, G\G, C\T, T\T, and G\T was 30%, 28%, 16%, 8%, 6%, and 12%, respectively, in patients with ALL and 33.33%, 26.67%, and 16.67%, respectively. 3.33%, 13.33%, and 6.67% in the group that was deemed to be in apparently healthy control (AHC). The frequency of the polymorphism's genotype distribution varied significantly between the patient and control groups. Conclusions: HHV6A and IL1R1 rs2234650 polymorphisms of ALL variations may be risk factors in the pathophysiology of ALL in Iraqi children.

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