Role of Human β-Defensin-3 in Rheumatoid Arthritis: An Observational Single-Center Study

Authors

  • Noor Ulhuda Ghazi Mohammed College of Science for Women, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0003-3549-1578
  • Faiq Isho Gorial Rheumatology Unit, Department of Medicine, College of Medicine, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0002-2760-5566
  • Fayhaa Magdad Khaleel Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0002-9951-2086
  • Baydaa Ahmed Abed National Diabetes Center, Mustansiriyah University, Baghdad, Iraq https://orcid.org/0000-0002-8895-7901
  • Samara Ali Mutar Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq
  • Layla Othman Farhan Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0002-5961-1338
  • Isam Noori Salman National Diabetes Center, Mustansiriyah University, Baghdad, Iraq

DOI:

https://doi.org/10.54133/ajms.v5i1S.289

Keywords:

Beta-human defensin-3, DAS28, Defensins, Pro-inflammatory cytokines, Rheumatoid arthritis

Abstract

Background: It's believed that HBD-3 is involved in the tissue remodeling process of articular cartilage. Also, HBD-3 has anti-inflammatory properties. Objectives: The purpose of this study is to assay human beta-defensine-3 (HBD-3) in serum from rheumatoid arthritis (RA) patients and investigate its correlation with proinflammatory cytokines. Methods: In this case-control study, fifty-eight RA patients were aged 20–65 years, and 29 age-matched healthy subjects (HS) had no inflammatory rheumatic diseases. The disease activity score-28 joint erythrocyte sedimentation rate (DAS28-ESR) was used to measure RA activity. CRP, ACPA, HBD-3, TNF-α, and IL-1β were assessed using the enzyme-linked immunosorbent assay technique (ELISA). Results: There was a significant increase in RF, ACPA, CRP, proinflammatory cytokines, and HBD-3 in the RA group compared with the HS group. There was no significant difference in HBD-3 levels according to the activity of diseases. The results of the correlation between HBD-3 and proinflammatory cytokines showed a significant positive relationship in the RA group. Conclusions: Inflammatory markers and S.HBD-3 demonstrated fair diagnostic performance to differentiate RA from HS. The current study supports the hypothesis that there is a correlation between HBD-3 and the immunoregulatory response.

Downloads

Download data is not yet available.

References

Santos CEM, Hurtado CNL, Santiago BR, Gonzalez-Amaro R, Cata YG, Fierro M de la LM, et al. LL-37, HNP-1, and HBD2/3 modulate the secretion of cytokines TNF-α, IL-6, IFN-γ, IL-10 and MMP1 in human primary cell cultures. Eur Cytokine Netw. 2016;27(3):68-74. doi: 10.1684/ecn.2016.0379.

Wani SS, Dar PA, Zargar SM, Dar TA. Therapeutic potential of medicinal plant proteins: present status and future perspectives. Curr Protein Pept Sci. 2020;21(5):443-487. doi: 10.2174/1389203720666191119095624.

Ghosh SK, McCormick TS, Weinberg A. Human beta defensins and cancer: contradictions and common ground. Front Oncol. 2019;9:341. doi: 10.3389/fonc.2019.00341.

Ramazi S, Mohammadi N, Allahverdi A, Khalili E, Abdolmaleki P. A review on antimicrobial peptides databases and the computational tools. Database. 2022;2022:baac011. doi: 10.1093/database/baac011.

Dhople V, Krukemeyer A, Ramamoorthy A. The human beta-defensin-3, an antibacterial peptide with multiple biological functions. Biochim Biophys Acta (Biomembranes). 2006;1758(9):1499-1512. doi: 10.1016/j.bbamem.2006.07.007.

Mohammed NUG, Khaleel FM, Gorial FI. The role of serum chitinase-3-like 1 protein (YKL-40) level and its correlation with proinflammatory cytokine in patients with rheumatoid arthritis. Baghdad Sci J. 2022;19(5):1014-1020. doi: 10.21123/bsj.2022.6293.

Farhan LO, Taha EM, Farhan AM. A Case control study to determine Macrophage migration inhibitor, and N-telopeptides of type I bone collagen Levels in the sera of osteoporosis patients. Baghdad Sci J. 2022;19(4):848-854. doi: 10.21123/bsj.2022.19.4.0848.

Liang W, Diana J. The dual role of antimicrobial peptides in autoimmunity. Front Immunol. 2020;11:2077. doi: 10.3389/fimmu.2020.02077.

Mahbub J. The Generating factors of antimicrobial peptide’s production in human. Theses submitted to Department of Pharmacy, BRAC University, Dhaka, Bangladesh; 2017.

Da Silva J, Leal EC, Carvalho E. Bioactive antimicrobial peptides as therapeutic agents for infected diabetic foot ulcers. Biomolecules. 2021;11(12):1894. doi: 10.3390/biom11121894.

Farhan LO, Abed BA, Dawood A. Comparison study between adipsin levels in sera of Iraqi patients with diabetes and neuropathy. Baghdad Sci J. 2023;20(3):726. doi: 10.21123/bsj.2022.7408.

Kashtl GJ, Abed BA, Farhan LO, Noori I, Salman ASD. A comparative study to determine LDH enzyme levels in serum samples of women with breast cancer and women with breast cancer and type 2 diabetes mellitus. Baghdad Sci J. 2023;20(3):883-890. doi: 10.26655/JMCHEMSCI.2023.4.20.

Hancock REW, Haney EF, Gill EE. The immunology of host defence peptides: beyond antimicrobial activity. Nat Rev Immunol. 2016;16(5):321-334. doi: 10.1038/nri.2016.29.

Mohammed AM, Zayni SM, AL-Anee MM, Corial FI, Al-Rubaee A. Diagnostic and predictive values of IL-6 in a group of Iraqi patients with rheumatoid arthritis. J Fac Med Baghdad. 2023;65(2). doi: 10.32007/jfacmedbagdad.2044.

Park O-J, Kim J, Ahn KB, Lee JY, Park Y-J, Kum K-Y, et al. A 15-amino acid C-terminal peptide of beta-defensin-3 inhibits bone resorption by inhibiting the osteoclast differentiation and disrupting podosome belt formation. J Mol Med. 2017;95(12):1315-1325. doi: 10.1007/s00109-017-1589-2.

Mohammed NUG, Khaleel FM, Gorial FI. Cystatin D as a new diagnostic marker in rheumatoid arthritis. Gene Rep. 2021;23:101027. doi: 10.1016/j.genrep.2021.101027.

Tago M, Sawada T, Nishiyama S, Tahara K, Kato E, Hayashi H, et al. Influence of large joint involvement on patient–physician discordance in global assessment of rheumatoid arthritis disease activity analyzed by a novel joint index. Int J Rheum Dis. 2018;21(6):1237-1245. doi: 10.1111/1756-185X.13281.

Varoga D, Wruck CJ, Tohidnezhad M, Brandenburg L, Paulsen F, Mentlein R, et al. Osteoblasts participate in the innate immunity of the bone by producing human beta defensin-3. Histochem Cell Biol. 2009;131(2):207. doi: 10.1007/s00418-008-0522-8.

Lee PH, Chen MY, Lai YL, Lee SY, Chen HL. Human beta-defensin-2 and-3 mitigate the negative effects of bacterial contamination on bone healing in rat calvarial defect. Tissue Eng Part A. 2018;24(7–8):653-661. doi: 10.1089/ten.tea.2017.0219.

Zhang L. Different dynamics and pathway of disulfide bonds reduction of two human defensins, a molecular dynamics simulation study. Proteins Struct Funct Bioinforma. 2017;85(4):665-681. doi: 10.1002/prot.25247.

Aletaha D, Neogi T, Silman AJ, Funovits J, Felson DT, Bingham CO, et al. 2010 Rheumatoid arthritis classification criteria: an American College of Rheumatology/European League Against Rheumatism collaborative initiative. Arthritis Rheum. 2010;62(9):2569-2581. doi: 10.1002/art.27584.

Paulsen F, Pufe T, Conradi L, Varoga D, Tsokos M, Papendieck J, et al. Antimicrobial peptides are expressed and produced in healthy and inflamed human synovial membranes. J Pathol. 2002;198(3):369-377. doi: 10.1002/path.1224.

Varoga D, Pufe T, Mentlein R, Kohrs S, Grohmann S, Tillmann B, et al. Expression and regulation of antimicrobial peptides in articular joints. Ann Anatomy. 2005;187(5–6):499-508. doi: 10.1016/j.aanat.2005.03.004.

Frasca L, Lande R. Role of defensins and cathelicidin LL37 in auto-immune and auto-inflammatory diseases. Curr Pharm Biotechnol. 2012;13(10):1882-1897. doi: 10.2174/138920112802273155.

Alvand A, Rezapoor M, Parvizi J. The role of biomarkers for the diagnosis of implant-related infections in orthopaedics and trauma. Adv Exp Med Biol. 2017;971:69-79. doi: 10.1007/5584_2017_11.

Bian T, Li H, Zhou Q, Ni C, Zhang Y, Yan F. Human β-defensin 3 reduces TNF-α-induced inflammation and monocyte adhesion in human umbilical vein endothelial cells. Mediators Inflamm. 2017;2017. doi: 10.1155/2017/8529542.

Moretta A, Scieuzo C, Petrone AM, Salvia R, Manniello MD, Franco A, et al. Antimicrobial peptides: A new hope in biomedical and pharmaceutical fields. Front Cell Infect Microbiol. 2021;11:668632. doi: 10.3389/fcimb.2021.668632.

Yan Y, Li Y, Zhang Z, Wang X, Niu Y, Zhang S, et al. Advances of peptides for antibacterial applications. Colloids Surfaces B Biointerfaces. 2021;202:111682. doi: 10.1016/j.colsurfb.2021.111682.

Downloads

Published

2023-11-02

How to Cite

Mohammed, N. U. G., Gorial, F. I., Khaleel, F. M., Abed , B. A., Mutar, S. A., Farhan , L. O., & Salman, I. N. (2023). Role of Human β-Defensin-3 in Rheumatoid Arthritis: An Observational Single-Center Study . Al-Rafidain Journal of Medical Sciences ( ISSN 2789-3219 ), 5(1S), S71–75. https://doi.org/10.54133/ajms.v5i1S.289

Most read articles by the same author(s)

Similar Articles

1 2 3 > >> 

You may also start an advanced similarity search for this article.