Determine the Whole Genome Sequences of SARS-COV-2 Isolated from Iraqi Patients Using NGS Method

Israa hashem Al-Zubaidy; Saife Al-Ahmer; Mohammed Issa Aldafaee

doi:10.54133/ajms.v6i2.777

Authors

Israa hashem Al-Zubaidy Institute of Genetic Engineering and Biotechnology for Post Graduate Studies, University of Baghdad, Baghdad,Iraq https://orcid.org/0000-0002-1393-2785
Saife Al-Ahmer Institute of Genetic Engineering and Biotechnology for Post Graduate Studies, University of Baghdad, Baghdad, Iraq
Mohammed Issa Aldafaee Medical City, Baghdad, Iraq

DOI:

https://doi.org/10.54133/ajms.v6i2.777

Keywords:

SARS corona virus-2, WGS, NGS

Abstract

Background: Next-generation sequencing (NGS) can monitor the transmission of COVID-19 and viral alterations. Objectives: To provide information about testing techniques and infection control measures, as well as to direct the development of vaccines and treatments for the Corona virus. Methods: Six Iraqi SARS-CoV-2 strains were investigated using whole-genome sequencing using the next-generation sequencing method. The sequencing was carried out with an Illumina MiSeq system, and phylogenetic analysis was carried out for all Iraqi sequences retrieved from GISAID. Results: The analysis of the isolates from this study showed that all the sequences from the most recent wave, which happened in the summer of 2022, were primarily clustered in the 20A clades and the 21K, 21L (Omicron) clades, as determined by the GISAID and Nextclade systems. On the other hand, the PANGO system revealed that six sequences were of the BA.1 lineage in Iraq, while four were of the BA.2 lineage. We found that throughout the country's subsequent pandemic waves, SARS-CoV-2 clades and their lineages exhibited circulation patterns and dominance. Conclusions: NGS continues to supply vital COVID-19 evidence to academics, vaccine and medication makers, and public health regulators.

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